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BPC-157

Status Not approved for human useBest evidence E4Sources checked 2026-09-17

BPC-157 is a synthetic fifteen amino acid peptide with no identified receptor after more than thirty years of study. About 77 percent of its 230 published papers come from one laboratory in Zagreb, and all three published human reports come from one clinic in Florida, totalling thirty-one patients with no control group.

Identity

Sequence
Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val (GEPPPGKPADDAGLV)
Formula
C62H98N16O22
Molar mass
1419.5 g/mol
CAS
137525-51-0
PubChem CID
9941957

What it is

BPC-157 is a synthetic chain of fifteen amino acids, sequence GEPPPGKPADDAGLV. It is almost always described as a fragment of a naturally occurring human protein, and that description does not survive checking.

The claim traces to one sentence in a 1993 paper from the group that named the compound, reporting a 40 kDa gastric protein isolated and a fifteen amino acid fragment characterised (Sikiric 1993). No publication reporting that protein isolation, purification or sequencing exists, and it has no UniProt or GenBank accession. The only primary description is a 1994 US patent, which an independent review found describes only crude chromatography and dialysis.

So the accurate description is narrower: a synthetic peptide claimed to correspond to a fragment of a protein nobody has characterised. Reviews published in 2025 and 2026 still call it naturally occurring.

The form sold as pentadeca arginate, or PDA, is the same sequence with arginine as the counter-ion instead of acetate. A salt, not a different peptide. No peer-reviewed study of it exists and the FDA review did not evaluate it.

How it works

No receptor has been identified, no binding affinity measured, and no knockout or knockdown experiment has demonstrated a required target. After roughly thirty-four years and about 230 papers, the molecular target is unknown. That constrains everything else on this page.

The vascular finding is the one that has held up

A group at Chang Gung University in Taiwan reported that BPC-157 raises VEGFR2 expression and activates a VEGFR2 to Akt to eNOS pathway, improving blood flow recovery in rat hindlimb ischaemia (Hsieh 2017), and separately reported nitric-oxide-mediated relaxation of isolated rat aorta (Hsieh 2020). In 2026 an unrelated Turkish group reproduced that relaxation in human arterial tissue, using internal mammary artery rings from twelve donors (Bilgic 2026).

Two unaffiliated groups, two species, one converging result. This is the only claim in the BPC-157 literature that meets an ordinary standard of independent replication.

Beyond that, the measured findings are mostly cell culture, and the broader framework claim, that BPC-157 works through a general interaction with the nitric oxide system, is asserted across dozens of papers from the originating group rather than demonstrated by any single experiment.

Research evidence

Human evidence: one clinic, thirty-one patients

Three published papers report giving BPC-157 to people and recording an outcome. All three share a first author and a journal.

  • A retrospective chart review of intra-articular injection for knee pain. Seventeen patients, sixteen reached by telephone, only twelve of those on BPC-157 alone, rating pain from memory six months to a year later. The paper states that no specific tools were used to measure function, quality of life or stiffness. No control group (Lee 2021).
  • A single-arm report in interstitial cystitis. Twelve women, no control group, 10 mg injected around the inflamed bladder area during cystoscopy, one procedure. Ten of twelve rated their symptoms fully resolved on a five-point subjective scale (Lee 2024).
  • An intravenous safety pilot in two people, 10 mg then 20 mg. The paper notes that both participants had already received intravenous BPC-157 before enrolling (Lee 2025).

The cystitis result deserves a second look rather than a citation. Cystoscopy with bladder distension is itself associated with temporary relief in interstitial cystitis, so in a single-arm design with a subjective endpoint the procedure and the compound cannot be separated. The paper does not raise this.

Across all three, roughly thirty-one patients, none with a control group, every outcome self-reported, and all of it from one physician in Orlando, Florida.

The two controlled studies that are rarely mentioned

Two earlier human studies had a placebo arm, and both are absent from most popular accounts. Neither was published in full, so neither has a PubMed record, and both are visible only through the FDA briefing document.

  • A randomised, placebo-controlled trial in mild to moderate ulcerative colitis. Fifty-three randomised, forty-six completed, 80 mg by rectal enema once daily for two weeks. Disease activity index change was minus 3.2 on BPC-157 against minus 1.6 on placebo, with confidence intervals that overlap. Conference abstract only.
  • A tolerability study in twenty-four healthy volunteers, rectal enema up to 2 mg/kg daily for eight days. The reported result: BPC-157 was not detected in plasma samples. Conference abstract only.

That second finding is the only human measurement of whether BPC-157 reaches the circulation by any route, and it sits awkwardly beside a compound taken for systemic effect. FDA called the randomised data inadequate to support efficacy or safety.

Preclinical evidence

The animal and cell literature is large and reports benefit across an unusually wide range of models: gastric and duodenal ulcer, colitis, oesophagitis, intestinal anastomosis, short bowel syndrome, several fistula models, Achilles transection, tendon-to-bone detachment, transected quadriceps, ligament transection, traumatic brain injury, spinal cord compression, cerebral ischaemia, thrombosis and portal hypertension.

The breadth is itself a finding. A compound with no identified receptor improving outcomes in nearly every tissue and nearly every injury model is a pattern that calls for a mechanism, and none has been offered that would predict it.

The concentration problem

Of 230 papers indexed in PubMed on BPC-157, about 176, roughly 77 percent, carry Predrag Sikiric of the University of Zagreb as an author. Around 143 of those are primary research reports from that group. Sven Seiwerth, the group pathologist, appears on 163 of the 230. Counting carefully through the remainder, about fifteen papers, roughly 6.5 percent of the corpus, are primary biological studies by a group with no connection to Zagreb or to Pliva, the Croatian pharmaceutical company that licensed the compound.

Thirty-plus years, dozens of journals, one laboratory. That is not an accusation. It is why the independent work matters more than its volume suggests.

The first independent test of the tendon claim

Tendon healing is the claim BPC-157 is best known for, and until 2026 no unaffiliated group had tested it in a live animal. In July 2026 a Turkish group transected and repaired the Achilles tendon in thirty-two rats, giving BPC-157 at 10 micrograms per kilogram per day intraperitoneally, the exact dose and route of the original Zagreb work, for four weeks (Bicer 2026).

BPC-157 did not reach statistical significance on maximum load to failure, on the total Bonar score, or on the total Movin score, and showed no significant difference in collagen type I expression. The authors describe numerically lower scores that did not reach significance. TB-500, included as a comparator in the same experiment, did reach significance on all three.

One study is one study, and a single negative result settles nothing that the positive results have not settled either. It is here because it is the first independent in vivo attempt at the flagship claim, at the originators own dose.

The dose that does not behave like a dose

The standard design in the Zagreb animal work gives three doses in the same experiment: 10 micrograms, 10 nanograms and 10 picograms per kilogram, a range spanning one million fold. Comparable benefit is reported at all three, and the group calls this an equipotent dosage, framing it as a property of the compound.

A receptor-mediated agent with a flat response across six orders of magnitude has no standard pharmacological interpretation. No independent group has tested it and no mechanistic account of it has been published.

The practical consequence matters more than the puzzle. Every dose conversion in circulation is built on those animal figures, and if 10 picograms per kilogram works as well as 10 micrograms, there is no dose to convert.

Safety

There is no human safety data at any duration beyond two weeks, by any route. The longest documented human exposure anywhere in the record is the two-week enema study described above.

The FDA gap list from the July 2026 advisory committee is specific. No acute toxicity study by any nominated route, meaning oral, rectal, subcutaneous or nasal. No repeat-dose toxicity by any of those routes, the only such data being a 28-day intramuscular set. No carcinogenicity study identified at all, which for an agent that raises VEGFR2 and promotes vessel growth is the most consequential absence on the list.

The 28-day intramuscular studies did produce signals: shortening and prolongation of activated partial thromboplastin time in rats and dogs, which FDA described as suggestive of altered clotting properties, together with raised ALT, glucose and triglycerides.

Immunogenicity has not been assessed in any species, and FDA said the risk could not be ruled out given aggregation potential and missing impurity testing. Three FAERS reports involve injectable BPC-157, including diffuse hyperpigmentation with gingival darkening that recurred on re-use.

The originating group states in its reviews that no toxicity has been observed and that a lethal dose could not be reached. FDA reviewed the same material and did not accept it as adequate.

Regulatory status

BPC-157 is not an approved drug anywhere. No approved products, no pending applications, and no entry in the European, Japanese or International Pharmacopoeias.

It was nominated for the 503A bulk drug substances list in 2015 and 2018, placed in Category 2 in 2023 for immunogenicity and impurity concerns, and removed from that category in April 2026 because the nominations had been withdrawn. Removal does not make a substance eligible for compounding.

On 23 July 2026 the Pharmacy Compounding Advisory Committee voted to recommend adding it, against FDA staff, eight to six with one abstention, though one secondary analysis records eight to seven. FDA reviewers had found no evidence of effectiveness for the nominated indication, ulcerative colitis, and no study using the oral, subcutaneous, nasal or transdermal route in those patients. FDA has not acted, the vote is non-binding, and rulemaking would come first.

BPC-157 is not named individually on the WADA prohibited list but falls under class S0, non-approved substances, by the category definition. It is detectable in urine along with five metabolites.

Handling and identity

FDA found BPC-157 not well characterised physically or chemically, partly because the naming is inconsistent. Body Protection, Body Protective and Body Protecting Compound all appear in peer-reviewed papers for the same substance, as do the codes PL-10, PLD-116, PL 14736 and AK-15. BPC-15 is a different compound, and papers treating it as BPC-157 evidence are over-reading.

Per FDA, the lyophilised free base keeps about three weeks at room temperature, storage recommended below minus 18 degrees; in solution, two to three weeks at 4 degrees and three to four months at minus 20. It is sensitive to pH and heat, which drives aggregation.

Elimination half-life in rats and dogs is under thirty minutes, intramuscular bioavailability 14 to 19 percent in rats and 45 to 51 percent in dogs. No oral or subcutaneous figure exists in any species, which is worth holding next to the claim that the arginate form is 90 percent orally bioavailable. That figure appears in no peer-reviewed paper.

What is not known

What BPC-157 binds to. No receptor has been identified, no binding affinity measured, and no knockout or knockdown experiment has shown a required target, after roughly 230 papers.

Whether it reaches the circulation in a human being. The only human measurement by any route found it undetectable in plasma. There is no human pharmacokinetic data after oral, subcutaneous, nasal or transdermal administration, and no absolute bioavailability figure for any route in any species other than intramuscular.

Why the animal dose-response is flat across a million-fold range, and what that implies about any conversion from animal doses to human amounts.

Whether it is carcinogenic. No carcinogenicity study has been identified, for a compound that raises VEGFR2 and promotes vessel growth. Also absent: acute toxicity by any route in common use, repeat-dose toxicity other than a 28-day intramuscular set, immunogenicity testing in any species, and reproductive toxicity outside the gestational period.

Whether it is safe beyond two weeks. There is no human safety data at any longer duration, by any route, and none for repeated cycles in any species.

Whether the flagship tendon result reproduces. Its first independent in vivo test, at the original dose and route, did not reach significance on any primary measure.

Anything at all about the arginate form sold as pentadeca arginate. No peer-reviewed study of it exists and the FDA review did not evaluate it.

Doses used in published research

Amounts administered under a trial protocol. Not dosing, not a recommendation.
TrialPopulationDose armsDurationWhat it measured
Veljaca 2002 and 200324 healthy volunteersUp to 2 mg/kg once daily, rectal enema8 daysSafety, and whether BPC-157 could be detected in plasma
Ruenzi 200553 randomised and 46 completers with mild to moderate ulcerative colitis80 mg once daily, rectal enema, against placebo2 weeksChange in the Disease Activity Index
Lee 2021 chart review17 charts reviewed, 16 patients reached, 12 of them on BPC-157 alone, chronic knee pain2 to 4 mg intra-articular injection, one or two dosesOne or two injections, no set coursePatient rated knee pain, recalled by telephone 6 to 12 months later
Lee 2024 pilot12 women aged 39 to 76 with interstitial cystitis, single arm10 mg total, injected around the inflamed bladder area during cystoscopyOne procedureGlobal Response Assessment, a five point subjective scale
Lee 2025 pilot2 adults, both of whom had already received intravenous BPC-157 before enrolling10 mg on day one then 20 mg on day two, intravenous infusion over 1 hour2 daysRoutine chemistry and vital signs, plus side effects the participants volunteered

Five human studies have administered BPC-157, and their amounts are on record. They do not agree with each other, they span three routes, and not one of them established a dose for anything.

By rectal enema: 80 mg once daily for two weeks in a randomised placebo-controlled trial in ulcerative colitis, and up to 2 mg/kg once daily for eight days in a healthy-volunteer tolerability study. Both are conference abstracts that were never published in full.

By intra-articular injection: 2 to 4 mg, one or two doses, in a retrospective chart review of knee pain.

By injection around the bladder during cystoscopy: 10 mg in a single procedure, in a twelve-patient single-arm report.

By intravenous infusion: 10 mg on day one and 20 mg on day two, in two people who had both already received intravenous BPC-157 before enrolling.

Note the range. From 80 mg rectally to 2 mg into a joint is a forty-fold spread across routes with no pharmacokinetic data to connect them, and the one study that looked for BPC-157 in plasma did not detect it.

Compiled from: Lee 2021, Alternative Therapies in Health and Medicine; Lee 2024, Alternative Therapies in Health and Medicine; Lee 2025, Alternative Therapies in Health and Medicine; FDA briefing document, Pharmacy Compounding Advisory Committee, July 2026

No dose has been established as safe or effective for any indication.

Amounts above record what a named source reported administering in a study. They are not a recommendation, not a protocol, and not instructions.

Converting a vial and a syringe into a draw volume is a separate, mechanical question from what amount to use. The reconstitution calculator does that arithmetic for peptide, HCG, and HGH vials.

What circulates E5

Figures reported online. Not dosing, not verified, not endorsed.
Reported useRouteAmount reportedFrequencyReported length
Injury and recoverySubcutaneous200 to 500 mcgOnce or twice daily4 to 8 weeks
Severe or post-surgicalSubcutaneous500 to 800 mcgOnce daily4 weeks
Gut complaintsOral250 mcg to 1 mgOnce daily4 to 6 weeks
Joint or localIntra-articular2 to 4 mgOne or two dosesNot specified
As part of the Wolverine blendSubcutaneousVaries by vendorNot standardised4 to 12 weeks

Circulating figures for BPC-157 are tightly clustered, and the injectable convention is the most consistent of any compound on this site: 250 micrograms once or twice daily, subcutaneous, in four to eight week blocks with a break in between. Weight-based versions appear as roughly 2.5 to 3.75 micrograms per kilogram twice daily, or a flat 10 micrograms per kilogram per day described as the animal reference standard.

That last phrase is where the arithmetic comes from, and it is worth following. The 10 micrograms per kilogram figure is real and does appear in the animal literature. What the conversion leaves out is that the same experiments administered 10 nanograms and 10 picograms per kilogram alongside it and reported comparable benefit at all three. The originating group calls this an equipotent dosage. A million-fold range producing the same result is not a dose-response curve, and a figure scaled from one end of it is not a translated dose.

The oral figures rest on a separate gap. Animal oral efficacy was reported at roughly 6 micrograms per kilogram per day delivered in drinking water, which is an efficacy observation rather than a bioavailability measurement. No oral bioavailability value exists for any species, and the 90 percent figure attached to the arginate form appears in no peer-reviewed paper.

Recorded as an observation about what is published elsewhere. No figure here is a dose, a protocol, or a recommendation, and nothing in this section is evidence that any amount is safe or effective.

Sources

  1. US Food and Drug Administration. BPC-157 (free base) and BPC-157 acetate. Briefing document, Pharmacy Compounding Advisory Committee meeting, 23-24 July 2026. FDA media 193343
  2. Sikiric P, et al. A new gastric juice peptide, BPC. An overview of the stomach-stress-organoprotection hypothesis and beneficial effects of BPC. J Physiol Paris. 1993. PMID 8298609
  3. Sikiric P, et al. Peptides having pharmaceutical activity. US Patent 5,288,708. 1994. US 5,288,708
  4. Hsieh MJ, et al. Therapeutic potential of pro-angiogenic BPC157 is associated with VEGFR2 activation and up-regulation. J Mol Med. 2017. PMID 27847966
  5. Hsieh MJ, et al. Modulation of vascular tone by BPC157 through Src-Caveolin-1-eNOS. Sci Rep. 2020. PMID 33051481
  6. Vasorelaxant effect of BPC-157 in human internal mammary artery. J Clin Med. 2026. PMID 42123221
  7. Chang CH, et al. The promoting effect of pentadecapeptide BPC 157 on tendon fibroblasts and the FAK-paxillin pathway. J Appl Physiol. 2011. PMID 21030672
  8. Comparison of BPC-157 and TB-500 on Achilles tendon healing in a rat model. Jt Dis Relat Surg. 2026. PMID 42542926
  9. Staresinic M, et al. Effective therapy of transected quadriceps muscle in rat: gastric pentadecapeptide BPC 157. J Orthop Res. 2003. PMID 14554208
  10. Cerovecki T, et al. Pentadecapeptide BPC 157 (PL 14736) improves ligament healing in the rat. J Orthop Res. 2010. PMID 20225319
  11. Pharmacokinetics and metabolism of BPC 157 in rats and beagle dogs. Front Pharmacol. 2022. PMID 36588717
  12. Cox HD, Eichner D. Detection of BPC-157 and its metabolites in human urine. Drug Test Anal. 2017. PMID 28035768
  13. Sikiric P, et al. Focus on ulcerative colitis: stable gastric pentadecapeptide BPC 157. Curr Pharm Des. 2013. PMID 22950504
  14. Lee E, Padgett B. Intra-articular injection of BPC 157 for multiple types of knee pain. Altern Ther Health Med. 2021. PMID 34324435
  15. Lee E, Walker C, Ayadi B. Effect of BPC-157 on symptoms in patients with interstitial cystitis: a pilot study. Altern Ther Health Med. 2024. PMID 39325560
  16. Lee E, Burgess K. Safety of intravenous infusion of BPC157 in humans: a pilot study. Altern Ther Health Med. 2025. PMID 40131143
  17. An independent review of the BPC-157 literature and patent record. Pharmaceuticals. 2025. PMID 40005999
  18. US Food and Drug Administration. Ipamorelin acetate, 503B, listed 29 September 2023, citing immunogenicity risk and serious adverse events including death reported in the literature after intravenous administration. FDA Category 2