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Semax

Status Registered as a prescription drug in Russia; not approved in the US, EU or other major jurisdictionsBest evidence E3Sources checked 2026-09-18

Semax is a Russian-developed ACTH(4-10) fragment analogue, registered as a nasal-drop nootropic drug in Russia and nowhere else. It has real published human trials in post-stroke rehabilitation, but none is a blinded, placebo-controlled study by Western standards, and none has been independently replicated outside Russia.

Identity

Sequence
Met-Glu-His-Phe-Pro-Gly-Pro (heptapeptide; ACTH(4-7) with a Pro-Gly-Pro tripeptide appended to the C-terminus)
CAS
80714-61-0, as commonly cited in the literature and on vendor material; not independently re-derived for this entry

What it is

Semax is a synthetic heptapeptide, Met-Glu-His-Phe-Pro-Gly-Pro, built from the first four residues of ACTH(4-10) with a Pro-Gly-Pro tripeptide added to the end. It was developed in the Soviet Union in the 1980s at what is now the Institute of Molecular Genetics in Moscow, and it is registered in Russia as a prescription nootropic drug, sold as intranasal drops. It has never been submitted for approval in the United States, the European Union, or any other major regulatory jurisdiction, and it has no FDA label, no EMA authorisation and no INN.

It is not thought to act through the classical ACTH receptor pathway that governs cortisol release; the Pro-Gly-Pro addition and truncation are specifically what removed the hormonal, adrenal-stimulating activity of the parent ACTH fragment while research groups reported retaining central nervous system effects. It is usually described as acting on the BDNF (brain-derived neurotrophic factor) system, though the mechanism is not settled.

The human trial record

Semax has been given to human beings in published, Russian-language clinical studies, which sets it apart from most compounds on this site. That is worth stating plainly rather than treating the Russian literature as equivalent to nothing. It is also worth stating exactly what kind of trial record this is.

A 1997 study gave Semax to 30 patients in the acute phase of hemispheric ischemic stroke, compared against 80 patients on conventional therapy, and reported the most effective daily doses as 12 milligrams for moderate strokes and 18 milligrams for severe strokes, each over a 5- to 10-day course. A separate 1999 study examined immunobiochemical markers in the acute stroke period and reported a shift toward anti-inflammatory cytokine activity. A larger 2018 study followed 110 post-stroke patients through early or late rehabilitation, with or without Semax, using a standard regimen of 6,000 micrograms per day for two 10-day courses separated by a 20-day interval, and reported that Semax raised plasma BDNF and was associated with better Barthel index outcomes.

None of these three studies is a blinded, placebo-controlled trial by the standard this site applies elsewhere. The 1997 and 2018 studies compared a Semax group against a non-randomised control group receiving standard care, not a placebo; whether patients, clinicians or outcome assessors were blinded is not established in the material reviewed for this entry. The 1997 study is formally labelled a controlled clinical trial in its indexing, but that label does not by itself establish blinding or randomisation. Every one of these studies was conducted at Russian institutions, published only in Russian-language journals, and none has been independently replicated outside Russia in the sources located here.

No trial of Semax appears on ClinicalTrials.gov, and no independent Western replication of the stroke findings was located for this entry.

What is not established

Whether the cognitive-enhancement, mood, focus and neuroprotection claims attached to Semax in consumer material, most of which trace to rodent and cell-culture work rather than to the stroke trials above, hold up in a healthy human being taking it for those purposes. The published human trials are specifically in post-stroke rehabilitation, not in healthy volunteers seeking cognitive enhancement, and the two populations should not be treated as interchangeable.

Whether the stroke findings would replicate in a blinded, placebo-controlled design. No such trial was located.

Long-term human safety data outside the stroke-rehabilitation context. No dedicated human toxicology, drug-interaction or long-term-use study was located.

What is not known

Whether the cognitive, mood, focus and neuroprotection claims made for Semax in consumer material hold up in healthy people; the published human trials are in post-stroke rehabilitation, not in healthy volunteers.

Whether the stroke findings would replicate under blinded, placebo-controlled conditions. No such trial was located.

Long-term human safety outside the stroke-rehabilitation setting. No dedicated toxicology or drug-interaction study was located.

The exact receptor or binding target through which Semax is thought to act. Its BDNF-elevating effect is repeatedly reported, but the upstream mechanism producing it is not settled in the sources reviewed.

Doses used in published research

Amounts administered under a trial protocol. Not dosing, not a recommendation.
TrialPopulationDose armsDurationWhat it measured
Gusev 1997 (PMID 11517472)30 patients, acute hemispheric ischemic stroke, vs. 80 patients on conventional therapyIntranasal Semax, 12 mg/day for moderate strokes or 18 mg/day for severe strokes5 to 10 daysClinical rating scales, EEG mapping, somatosensory evoked potentials
Gusev 2018 (PMID 29798983)110 patients after ischemic stroke, early or late rehabilitation, with or without SemaxIntranasal Semax, 6,000 mcg/day, two 10-day courses with a 20-day interval2 courses of 10 days, 20 days apartPlasma BDNF, motor performance (British Medical Research Council scale), Barthel index

Both trials compared Semax against standard rehabilitation care, not a placebo, and blinding is not established in the material reviewed for this entry. Neither is a Western-standard randomised, blinded, placebo-controlled trial, and neither has been independently replicated outside Russia.

Compiled from: Gusev 1997, Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova

No dose has been established as safe or effective for any indication.

Amounts above record what a named source reported administering in a study. They are not a recommendation, not a protocol, and not instructions.

Converting a vial and a syringe into a draw volume is a separate, mechanical question from what amount to use. The reconstitution calculator does that arithmetic for peptide, HCG, and HGH vials.

What circulates E5

Figures reported online. Not dosing, not verified, not endorsed.
Reported useRouteAmount reportedFrequencyReported length
Cognitive/nootropic use, generalIntranasal300 to 1,000 mcg per dose (a few drops of a 0.1% or 1% solution)One to three times dailyOften cycled, days to a few weeks

Circulating cognitive-enhancement material describes Semax at doses well below the stroke-trial figures above: typically a few hundred micrograms to about a milligram per dose, intranasally, against the 12 to 18 milligrams per day used in the published stroke rehabilitation trials. The circulating figure is roughly an order of magnitude below what has actually been studied in a human being, and that human study was in acute stroke, not in a healthy person seeking a cognitive effect.

No circulating source reviewed for this entry cites the stroke trials by name or dose when presenting a cognitive-use figure, which means the two are not being knowingly scaled from one another; they simply coexist under the same compound name.

Recorded as an observation about what is published elsewhere. No figure here is a dose, a protocol, or a recommendation, and nothing in this section is evidence that any amount is safe or effective.